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KMID : 1033620230500020107
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Clinical and Experimental Reproductive Medicine
2023 Volume.50 No. 2 p.107 ~ p.116
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Measurement of serum anti-Mullerian hormone by revised Gen II or automated assay: Reproducibility under various blood/serum storage conditions
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Lee Joong-Yeup
Kim Chung-Hyon
Choe Seung-Ah
Seo So-Yeon
Kim Seok-Hyun
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Abstract
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Objective : We investigated the agreement between anti-Mullerian hormone (AMH) levels measured with revised Gen II (rev-Gen II) and automated AMH (Access) assays and evaluated the reproducibility of each method under various blood/serum storage conditions.
Methods : AMH levels in blood samples from 74 volunteers were measured by rev-Gen II and Access assays under various conditions: immediate serum separation and AMH measurement (fresh control); serum stored at ?20 ℃ and AMH measured after 48 hours, 1 week, and 2 years; serum stored at 0 to 4 ℃ and AMH measured after 48 hours and 1 week; and blood kept at room temperature and delayed serum separation after 48 hours and 1 week, with immediate AMH measurement.
Results : In fresh controls, all rev-Gen II-AMH values were higher than comparable Access-AMH values (difference, 8.3% to 19.7%). AMH levels measured with the two methods were strongly correlated for all sample conditions (r=0.977 to 0.995, all p<0.001). For sera stored at ?20 ℃ or 0 to 4 ℃ for 48 hours, Access-AMH values were comparable to control measurements, but rev-Gen II-AMH values were significantly lower. AMH levels in sera stored at ?20 ℃ or 0 to 4 ℃ for 1 week were significantly lower than in fresh controls, irrespective of method. Across methods, long-term storage at ?20 ℃ for 2 years yielded AMH measurements significantly higher than control values. When serum separation was delayed, rev-Gen II-AMH values were significantly lower than control measurements, but Access-AMH values varied.
Conclusion : The rev-Gen II and Access-AMH assays showed varying reproducibility across blood/serum storage conditions, but automated Access yielded superior stability to rev-Gen II.
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KEYWORD
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Anti-Mullerian Hormone, Automation, Biological assay, Blood, Reproducibility of results, Serum
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